Figure 7.

Proposed model of Agarivorans sp. B2Z047 for alginate utilization. Based on the signal peptide, the lipoprotein signal peptide and the subcellular localization predictions, all 12 alginate lyases were displayed in the specific location of the bacterial cell. The enzymatic activity of each alginate lyase was from the function prediction and the degradation products assay of alginate lyase overexpressed in E. coli BL21 (DE3). Porin (KdgMN), oligoalginate transporters, DEH reductase (DehR) and ED pathway were annotated in the genome of strain B2Z047 (Table S3). The abbreviations are as follows: Aly7A proteins, Aly7A1, Aly7A2, Aly7A3, Aly7A4, and Aly7A5; Oal17A proteins, Oal17A1 and Oal17A2; DEH, 4-deoxy-L-erythro-5-hexoseulose uronic acid; KDG, 2-keto-3-deoxy-gluconate; ED pathway, Entner–Doudoroff pathway.