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. 2022 Mar 23;10(4):754. doi: 10.3390/biomedicines10040754

Figure 5.

Figure 5

Markers of inflammation in plasma after CER-001 injections. The levels of IL-1b, IL-6, and IL-8 were quantified from 1:2 diluted plasma using Luminex xMAP technology. Cytokine concentrations were determined using the Human Custom ProcartaPlex 12-plex kit. The method is detailed in Supplementary File S1. (a) Western blot analysis of acute phase proteins in the patient’s plasma. Plasma was diluted 1:40,000, separated to constant volume (8 μL), and analyzed with antibodies specific for C-reactive protein (CRP) and serum amyloid A-1 (SAA-1) after blotting. (b) Positive correlation between CRP and SAA-1 after quantification of Western blots (r = 0.93, p < 0.0001, Pearson correlation). IL-1b (c), IL-6 (d), and IL-8 (e) concentrations were determined by Bioplex from 1:2 diluted plasma.