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. 2022 Apr 11;11(4):582. doi: 10.3390/biology11040582

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of ERK2 in HSC-5^NW cells restored cell proliferation and stabilized nuclear FOXO1. (A) The graph shows the total cell number after 5 days of incubation of HSC-5^CN and HSC-5^NW cells treated with 2 nM Pyrazolyl or DMSO. (B) Representative immunofluorescence images of HSC-5^CN and HSC-5^NW cells treated as in (A) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. (C) Quantification of nuclear FOXO1 fluorescence from 20 randomly chosen cells per experiment and normalized to DMSO-treated HSC-5^CN cells. All values are the mean ± SD, n = 3, * p < 0.05, *** p < 0.001.