Figure 3.

Hierarchical clustering and difference between the two groups (T vs. N). (A) The hierarchical clustering heat-map for the tsRNAs. The color in the panel represents the relative expression level (log2-transformed). Blue represents an expression level below the mean, and red represents an expression level above the mean. (B) The scatter plots of differentially expressed tsRNAs. tsRNAs above the top line (red dots, upregulation) or below the bottom line (green dots, downregulation) show more than 1.5-fold change between the two compared groups. Gray dots indicate nondifferentially expressed tsRNAs. (C) The volcano plots of significantly differentially expressed tsRNAs. The values of the X and Y axes in the volcano plot were log2-transformed fold change and -log10-transformed p-values between the two groups, respectively. Red/green circles indicate statistically significant differentially expressed tsRNAs with a FC of no less than 1.5 and a p-value < 0.05 (red: upregulated; green: downregulated). Gray circles indicate nondifferentially expressed tsRNA with FC and/or q-value, which did not meet the cutoff thresholds.