Table 7.

Effect of dietary Ca level on protein expression levels of Ca metabolism-related enzymes and proteins in tibia bone of 42-d-old broilers ¹

Dietary Ca, %	ALP, RQ	OPG, RQ	BMP-2, RQ	OC, RQ	SOST, RQ	DMP-1, RQ	TRACP, RQ
0.5	0.781	0.614	0.774	0.739	0.520	0.618	0.588
0.6	0.810	0.795	0.980	0.704	0.643	0.617	0.499
0.7	1.002	0.931	0.978	0.723	0.657	0.958	0.899
0.8	0.865	0.950	1.002	0.930	0.682	0.716	0.626
0.9	1.003	0.815	1.011	0.647	0.922	0.749	0.644
1.0	1.043	0.955	1.010	0.922	0.730	0.765	0.977
1.10	0.707	0.808	0.857	1.010	0.987	1.000	1.052
SEM	0.1470	0.1719	0.1644	0.2188	0.2403	0.2103	0.2415
P-value	0.61	0.82	0.92	0.86	0.85	0.80	0.47

The protein levels were calculated as the relative quantity (RQ) of the target gene protein to the β-actin or β-tubulin protein.

Data represent the means of 6 replicates (n = 6).

ALP, alkaline phosphatase; OPG, osteoprotegerin; OC, osteocalcin; DMP-1, dentin matrix protein-1; BMP-2, bone morphogenetic protein-2; SOST, sclerostion; TRACP, tartrate-resistant acid phosphatase.