Figure 3.

Effect of metallothionein on PPE-induced acute lung injury. (A,B) Wildtype (WT) and MT-1 and -2 gene knockout (MT-KO) mice were treated with sterile saline (Cont) or PPE (10 U/kg) only once, on day 0. BALF samples were collected 72 h after the intratracheal administration of saline or PPE. (A) BALF cells were deposited onto slides by using a Cytospin^® 4 cytocentrifuge then stained with Diff-Quik reagents and visualized via light microscopy (scale bar, 100 µm). (B) The numbers of total cells, macrophages, and neutrophils were determined. The amount of protein present in the BALF was determined by the Bradford method. Values are the mean ± S.E.M. * or # p < 0.05; ** p < 0.01 (* or **, vs. Control; #, vs. WT). The experiments that produced the data shown in this figure were performed at least twice.