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. 2022 Apr 15;12(4):583. doi: 10.3390/biom12040583

Figure 4.

Figure 4

Effect of metallothionein on PPE-induced pulmonary inflammatory response. Wildtype (WT) and MT-1 and -2 gene knockout (MT-KO) mice were treated with sterile saline (Cont) or PPE (10 U/kg) only once, on day 0. Total RNA was extracted from the lungs 72 h after PPE administration and subjected to real-time RT-PCR using a specific primer set for each gene. Values were normalized to Gapdh and are expressed relative to the Control. Values are the mean ± S.E.M. * p < 0.05; ** or ## p < 0.01 (* or **, vs. Control; # or ##, vs. WT). The experiments that produced the data shown in this figure were performed at least twice.