Figure 3.

B. dothidea growth on calli. (a) Apple calli inoculated with B. dothidea. The 2-week-old calli were used for inoculation. The inoculation was performed by placing the mycelial discs on the center of the calli. The scale bars represent 9.0 mm. WT: wild type; KO: mutated calli generated by CAS9/CRIPR system. EV1: empty vector for gene editing; GC: genetic complemented calli. KO+EV2: KO calli transformed with empty vector for genetic complement. (b) Fungal growth evaluated by measuring the diameter of fungal plates. The bars represent means (± SD) from five calli dishes (n = 5). **, p < 0.01.