Figure 11.

In the immunohistochemical analyses, the C-6 treatment decreases the levels of protein aggregates and phosphorylated Ser-129-α-synuclein in the striatum of PFF-injected mice. (A) Based on fluorescent co-labeling in immunohistochemistry, PFF injection enhanced the levels of protein aggregates in Thioflavin-T stain and phosphorylated Ser-129-α-synuclein, whereas the C-6 treatment substantially reduced both labeling intensities in the striatum. (B,C) In the analysis of Thioflavin-T label intensity, both 5 mg/kg and 25 mg/kg treatments significantly reduced the label intensity of protein aggregates (B) and phosphorylated Ser-129-α-synuclein (C) in the striatum. One-way ANOVA with Dunnett’s test was adopted to compare the intensity of Thioflavin-T stain and phospho-Ser129-α-synuclein label present across the groups with or without treatment (#) (n = 7–8/group). **: p < 0.01 and ****: p < 0.0001.