TABLE 1.

PCR primers and conditions

PCR product	Primer sequence (5′-3′)a	Cycle no.	Anneal temp (°C)
Eotaxin	fwd-5′ ACATGAAGGTCT CCGCAGCACTTC	35	61
	rev-5′ TTGGCCAGGTTAAA GCAGCAGGTG
GM-CSF	fwd-5′ ATTGCGGCCCC GCCTGGAGCTGTACAAG	35	55
	rev-5′ ATTCTCGAGAC TGGCTCCCAGCAGTCAAA
RANTES	fwd-5′ GGCACGCCTCG CTGTCATCCTCA	35	65
	rev-5′ CTTGATGTGGG CACGGGGCAGTG
GAPDH	fwd-5′ ACCACAGTCCAT GCCATCA	35	55
	rev-5′ TCCACCACCCTG TTGCTGTA

^afwd, forward; rev, reverse.