Table 4.
Effect of salivary, gastric and intestinal in vitro digestion model on the phenolic content of VCS mg/g.
| Rt (min) | Compound | VCS Microencapsulated | VCS Solution | ||||||
|---|---|---|---|---|---|---|---|---|---|
| BD | SSF | SGF | SIF | BF | SSF | SGF | SIF | ||
| 3.85 | Quinic acid | N.D. | N.D. | N.D. | 0.84 ± 0.01 | N.D. | N.D. | N.D. | 6.56 ± 0.05 |
| 10.39 | Protocathecuic acid | N.D. | N.D. | N.D. | 0.72 ± 0.01 | N.D. | N.D. | N.D. | 6.44 ± 0.05 |
| 12.28 | 5-Caffeoylquinic acid (Chlorogenic acid) |
3.85 ± 0.02 a,b | 3.55 ± 0.02 b | 1.85 ± 0.01 c | 0.12 ± 0.01 d | 33.63 ± 0.23 a | 29.85 ± 0.19 b | 16.38 ± 0.11 c | 0.72 ± 0.01 d |
| 16.28 | Quercetin-glucoside | 0.24 ± 0.01 a,b | 0.20 ± 0.01 b | 0.14 ± 0.01 c | 0.00 | 1.86 ± 0.02 a,b | 1.78 ± 0.02 b | 0.86 ± 0.01 c | N.D. |
| Total Phenolics | 4.09 ± 0.03 a,b | 3.75 ± 0.02 b | 1.99 ± 0.01 c,d | 1.68 ± 0.01 d | 35.49 ± 0.25 a | 31.63 ± 0.20 b | 17.24 ± 0.10 c | 13.72 ± 0.09 d | |
Values are expressed as mean values ± SD, mg/g, n = 3. In the same row, for each type of V. corymbosum L. sample (microencapsulated and solution/aqueous extract), values marked with different letters (a–d) indicate a significant difference (p < 0.05) between non-digested and after each phase of digestion (One-way analysis of variance (ANOVA); multiple comparison test; Tukey multiple range test (p = 0.05); GraphPad Prism Version 8.0.1, Graph Pad Software, Inc., San Diego, CA, USA), VCS (V. corymbosum L.), BD—before digestion, SSF—Simulated salivary fluid, SGF—Simulated gastric fluid, SIF—simulated intestinal fluid.