Skip to main content
. 2022 Mar 24;8(4):336. doi: 10.3390/jof8040336

Figure 2.

Figure 2

GAG but not Pel is required for the adherence of P. aeruginosa to A. fumigatus. Confocal microscopy images of green fluorescent protein (GFP)-producing A. fumigatus co-cultured with mCherry-producing P. aeruginosa. (a) Wild-type P. aeruginosa (PA14) adherent to hyphae of wild-type (WT) A. fumigatus. (b) Pel-deficient P. aeruginosa (ΔpelA) adherent to hyphae of WT A. fumigatus. (c) Wild-type P. aeruginosa (PA14) not adherent to hyphae of GAG-deficient A. fumigatus (Δuge3). (d) Pel-deficient P. aeruginosa (ΔpelA) not adherent to hyphae of GAG-deficient A. fumigatus (Δuge3). (e) Pel-overexpressing ΔwspFΔpslPBADpel P. aeruginosa (PBADpel+) adherent to hyphae of WT A. fumigatus. (f) Pel-overexpressing ΔwspFΔpslPBADpel P. aeruginosa (PBADpel+) not adherent to hyphae of GAG-deficient A. fumigatus. Representative images of at least 2 independent experiments. Imaged at 630× (scale bar, 20 μm).