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. 2022 Mar 24;8(4):336. doi: 10.3390/jof8040336

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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A. fumigatus-secreted products increase the formation of P. aeruginosa-adherent biofilms. Crystal violet quantification of adherent biofilm biomass by (a) wild-type P. aeruginosa (PA14) and (b) Pel-deficient P. aeruginosa (ΔpelA) grown in the presence of media control, wild-type A. fumigatus culture supernatants (WT CS), or GAG-deficient A. fumigatus culture supernatants (Δuge3 CS). Bars represent the means ± standard deviations of the destain solution measured at 600 nm of 3 independent experiments. A significant increase in absorbance is indicated by * (p < 0.0001) relative to all bars as determined by one-way ANOVA with Tukey’s multiple-comparison test.