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. 2022 Mar 24;8(4):336. doi: 10.3390/jof8040336

Figure 5.

Figure 5

De-N-acetylated GAG is required to increase adherent biofilm formation by Pel-deficient P. aeruginosa. Adherent biofilm formation by Pel-deficient P. aeruginosa (ΔpelA) grown in the presence of de-N-acetylated GAG from WT de-N-acetylated GAG-containing CS (WT CS), 130 nM recombinant Agd3 deacetylase enzyme (rAgd3) or N-acetylated GAG from N-acetylated-GAG-containing culture supernatants (Δagd3 CS), or de-N-acetylated GAG from a combination of N-acetylated-GAG-containing culture supernatants and 130 nM rAgd3 (Δagd3 CS + rAgd3) was quantified with crystal violet staining. Bars represent the means ± standard deviations of 3 independent experiments. A significant increase in absorbance is indicated by * (p < 0.0001) relative to all bars as determined by one-way ANOVA with Tukey’s multiple-comparison test.