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. 2022 Mar 24;8(4):336. doi: 10.3390/jof8040336

Figure 8.

Figure 8

Inhibitory effects on the growth of A. fumigatus by secreted products within P. aeruginosa culture supernatants that dominate over the augmentation of A. fumigatus-adherent biofilm formation by secreted Pel. Adherent biofilm formation by (a) wild-type A. fumigatus (AF) (left) and GAG-deficient A. fumigatus hyphae (Δuge3) (right) grown in the presence of culture supernatants (CS) from wild-type A. fumigatus (AF), Pel-overexpressing ΔwspFΔpslPBADpel P. aeruginosa (PBADpel+), Pel-nonexpressing ΔwspFΔpslPBADpel P. aeruginosa (PBADpel-), or Pel-deficient P. aeruginosa (ΔpelA). (b) GAG-deficient A. fumigatus hyphae (Δuge3) grown in the presence of dialyzed culture supernatants (DCS) from wild-type A. fumigatus (AF), Pel-overexpressing ΔwspFΔpslPBADpel P. aeruginosa (PBADpel+), Pel-nonexpressing ΔwspFΔpslPBADpel P. aeruginosa (PBADpel−), wild-type Pel-dominant P. aeruginosa (PA14), or Pel-deficient P. aeruginosa (ΔpelA) was quantified with crystal violet staining. Bars represent the means ± standard deviations of the destain solution measured at 600 nm of at least 3 independent experiments. A significant decrease or increase in absorbance is indicated by * (p < 0.01), and no significant difference in absorbance is indicated by ns relative to cultures grown in media alone as determined by one-way ANOVA with Dunnett’s multiple-comparison test.