Table 1.

Validation criteria for analytical HPLC methods.

Antibiotic	Calibration Range (µg/mL)	R2	Intra-Day Precision [min; max] (%)	Inter-Day Precision [min; max] (%)	Limit of Detection [µg/mL]	Cochran’s Test Cexp	ANOVA (Non-Linearity) Fexp
Amoxicillin	120–280	0.9999	[0.06; 0.66]	[0.57–0.75]	0.13	0.344	0.15
Aztreonam	50–150	0.9997	[0.08; 1.48]	[1.18; 1.95]	0.51	0.399	0.93
Cefazolin	75–175	0.9999	[0.15; 0.85]	[0.44; 0.57]	0.11	0.609	0.38
Cefepime	60–140	0.9999	[0.04; 0.83]	[1.15; 1.70]	0.36	0.269	0.13
Cefiderocol	25–75	0.9999	[0.05; 1.53]	[0.41; 0.99]	0.10	0.424	0.07
Cefotaxime	50–150	0.9998	[0.08; 1.81]	[1.09; 1.66]	2.02	0.600	1.93
Cefoxitin	75–175	0.9993	[0.17; 2.04]	[1.40; 1.80]	0.53	0.420	0.66
Ceftazidime	100–500	0.9999	[0.02; 1.53]	[0.28; 0.94]	0.23	0.533	3.06
Ceftazidime/Avibactam	100–500	0.9999	[0.24; 0.50]	[0.31; 1.05]	2.43	0.551	0.21
	25–125	0.9999	[0.13; 0.43]	[0.33; 0.72]	3.17	0.514	1.38
Ceftozolane/Tazobactam	50–250	0.9999	[0.07; 1.93]	[0.62; 1.47]	0.77	0.579	0.26
	25–125	0.9999	[0.06; 2.04]	[0.63; 1.60]	0.84	0.643	0.49
Cloxacillin	1200–2800	0.9981	[0.33; 1.81]	[1.25; 1;95]	4.26	0.575	0.92
Meropenem	50–250	0.9999	[0.06; 1.30]	[0.71; 1.13]	0.19	0.659	0.64
Piperacillin	100–300	0.9999	[0.01; 0.46]	[0.55; 1.10]	0.34	0.555	0.06
Piperacillin/Tazobactam	100–500	0.9999	[0.12; 1.33]	[0.38; 1.62]	0.23	0.599	0.99
	12.5–62.5	0.9999	[0.01; 1.28]	[0.76; 1.44]	0.36	0.554	0.14
Temocillin	50–250	0.9999	[0.05; 1.72]	[0.91; 2.02]	0.64	0.331	0.04
Vancomycin	50–150	0.9995	[0.03; 1.65]	[1.70; 2.48]	2.91	0.389	2.27