Table 4.
Folate Vitamer | RT | m/z | Mean Value | Chemical Structure |
---|---|---|---|---|
THF C19H23N7O6 |
1.98 | 446.1783 | 22.40 ± 0.44 | |
5FTHF C20H23N7O7 |
2.44 | 474.1732 | 16.98 ± 0.46 | |
FA C19H19N7O6 |
3.35 | 442.147 | 16.57 ± 0.23 | |
5MTHF C20H25N7O6 |
3.42 | 460.1939 | 13.82 ± 0.13 | |
Total Folate content | 69.77 ± 0.45 |
THF: 5,6,7,8-tetrahydrofolic acid; 5FTHF: 5-Formyl-5,6,7,8-tetrahydrofolic acid; FA: folic acid; 5MTHF: 5-methyl-5,6,7,8-tetrahydrofolic acid.