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Challenges in finding tiny metastatic foci in gigapixel H–E WSIs. The segmentation of tiny micro metastases (denoted by red circles in (a) an H–E slide and (b) the associated IHC slide) is as challenging as finding a needle in a haystack. (a) Even in the high-magnification H–E image, ITCs are difficult to locate in humans. (b) In the high-magnification IHC image, metastases are visible as brown spots.
