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. 2022 Apr 15;9(4):190. doi: 10.3390/vetsci9040190

Table 1.

PCR primers used in this study.

Gene Primer (5′-3′) Amplicon Reference
PoIFN-β a Sense ATGGCTAACAAGTGCATCC 561 This study
Anti-sense TCAGTTCCGGAGGTAATCT
PoIFN-β b Sense AAATCGCTCTCCTGATGTGTT 539 This study
Anti-sense TCAGTGGTGGTGGTGGTGGTGGTTCCGGAGGTAATCTGTAA
PoIFN-β c Sense ATGTCAGAAGCTCCTGGGACAGTT 246 Sang et al.
Anti-sense AGGTCATCCATCTGCCCATCAAGT
PoIFN-β R Sense AGTGTTGGATGAATGCTAACAA 259 This study
Anti-sense TGGTGGAGAAACACATCAGG
GAPDH Sense TGGYATCGTGGAAGGRCTCAT 370 Sang et al.
Anti-sense RTGGGWGTYGCTGTTGAAGTC

a Primer pair for gene cloning; b Primer pair for expression vector construction; c Primer pair for detection of differential expression by real-time quantitative RT-PCR; R Primer pair for cloning of regulatory element of PoIFN-β gene.