Table 1.
Gene | Primer (5′-3′) | Amplicon | Reference | |
---|---|---|---|---|
PoIFN-β a | Sense | ATGGCTAACAAGTGCATCC | 561 | This study |
Anti-sense | TCAGTTCCGGAGGTAATCT | |||
PoIFN-β b | Sense | AAATCGCTCTCCTGATGTGTT | 539 | This study |
Anti-sense | TCAGTGGTGGTGGTGGTGGTGGTTCCGGAGGTAATCTGTAA | |||
PoIFN-β c | Sense | ATGTCAGAAGCTCCTGGGACAGTT | 246 | Sang et al. |
Anti-sense | AGGTCATCCATCTGCCCATCAAGT | |||
PoIFN-β R | Sense | AGTGTTGGATGAATGCTAACAA | 259 | This study |
Anti-sense | TGGTGGAGAAACACATCAGG | |||
GAPDH | Sense | TGGYATCGTGGAAGGRCTCAT | 370 | Sang et al. |
Anti-sense | RTGGGWGTYGCTGTTGAAGTC |
a Primer pair for gene cloning; b Primer pair for expression vector construction; c Primer pair for detection of differential expression by real-time quantitative RT-PCR; R Primer pair for cloning of regulatory element of PoIFN-β gene.