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. 2022 Apr 13;23(8):4281. doi: 10.3390/ijms23084281

Figure 4.

Figure 4

Estimation of apoB production by Huh7 cell lines. Wild-type (WT) cells and stable cell lines carrying FHBL causative variant in heterozygous state (He-KO) or homozygous state (Ho-KO), or carrying variant of interest in homozygous state (Ho-Leu351Arg), were cultured with oleic acid (OA) 0.6 mmol/L, complexed to BSA, for 0, 2 and 6 h. The apoB-100 concentrations in the lysate were measured by ELISA and were normalized to the protein concentrations in the cell lysate. The WT cell line 6 h after incubation with OA was considered as the reference for data normalization. * p < 0.05 (Welch’s t-test). Error bars, ±S.D. (n = 3).