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Up to 8 samples can be sequenced in the same run for the NextSeq 500/550 Mid Output v2.5 kit and 24 samples for the NextSeq 500/550 High Output v2.5 kit, ensuring a minimum of 17.5 million passing filter clusters per sample and a coverage of 97.7% with a depth of 200×, or 99.3% with a depth of 100×.
