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. 2022 Apr 14;20(4):267. doi: 10.3390/md20040267

Figure 1.

Figure 1

(A) The chemical structure of ATX and experimental procedure. ATX is dissolved in saline and orally administered once a day for three days before IR induction. The gerbils are sacrificed on day 1, 2 and 5 after IR; (B) Representative photographs of Nissl staining in the hippocampus (CA1-3) of the vehicle-treated (upper low) and ATX-treated (lower low) groups in sham (a,e), and on day 1 (b,f), day 2 (c,g), and day 5 (d,h) after IR. In the vehicle-IR group, CV stainability in the SP of CA1 (arrows in (c)) is pale at two days after IR, and, at five days after IR, very pale CV stainability is shown in the SP of CA1-3 (black asterisks in (d)). In the ATX-IR group, pale CV stainability is shown only in the SP of CA1 (white asterisk in (h)) at five days after IR. DG, dentate gyrus; SO, stratum oriens; SR, stratum radiatum. Scale bar = 400 μm.