Table 1.
Primary and secondary antibodies for immunohistochemical staining.
Primary Antibodies | Dilutions | Suppliers |
---|---|---|
Mouse anti-neuronal nuclei (NeuN) |
1:1000 | Chemicon, Temecula, CA, USA |
Goat anti-8-hydroxydeoxyguanosine (8OHdG) | 1:500 | Millipore, Billerica, MA, USA |
Mouse 4-hydroxy-2-nonenal (4HNE) |
1:800 | Alexis Biochemicals, San Diego, CA, USA |
Sheep anti-Cu, Zn-superoxide dismutase (SOD1) | 1:800 | Calbiochem, La Jolla, CA, USA |
Sheep anti-Mn-superoxide dismutase (SOD2) | 1:800 | Calbiochem, La Jolla, CA, USA |
Secondary Antibodies | Dilutions | Suppliers |
Biotinylated horse anti-mouse IgG | 1:250 | Vector Laboratories Inc., Burlingame, CA, USA |
Biotinylated horse anti-goat IgG | 1:250 | Vector Laboratories Inc., Burlingame, CA, USA |
Biotinylated rabbit anti-sheep IgG | 1:250 | Vector Laboratories Inc., Burlingame, CA, USA |