Figure 6.

In vitro activation of platelets in plasma results in detectable secretion of miRNAs. (A) Experimental setup of in vitro experiments performed to investigate the release of miRNAs upon platelet activation in PRP. Two different agonists were used: 6.5 μM ADP and 1 μg/mL 1A5. Addition of an equal volume of 0.9% NaCl served as unstimulated control. PPP samples were used directly for RNA isolation (total PPP) or as input for SEC. (B) miRNA levels in total PPP and the vesicle and protein fractions (n = 5). qPCR data were RNA spike-in normalized. Black dots indicate control samples, red dots indicate stimulation with 1A5, and blue dots signify platelet activation using ADP. The mean ± SD are plotted. Repeated-measures one-way ANOVA with Sidak’s multiple comparison test was calculated. In case of missing values, a mixed-effects analysis was performed. p-Values < 0.05 were considered significant. p-Values between 0.05 and 0.2 are depicted. * = p ≤ 0.05.