Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 11;23(8):4239. doi: 10.3390/ijms23084239

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

CLSM and TEM image of M-MWCNT uptake by RAW267.4. (a) TEM micrographs of M-MWCNT (indicated by arrow) internalized by macrophages. M-MWCNT was co-cultured with macrophages for 12 h and then the collected cells were immediately fixed with 2.5% glutaraldehyde. The fixed cells were sectioned and observed by TEM. (b) CLSM image of FITC-OVA uptake by RAW267.4. Macrophages were cultured with M-MWCNT+FITC-OVA or FITC-OVA for 24 h. The slides were fixed and stained with DAPI as indicated by blue fluorescence, while green fluorescence indicates FITC-OVA. The cells were observed and photographed with a laser confocal scanning microscope.

CLSM and TEM image of M-MWCNT uptake by RAW267.4. (a) TEM micrographs of M-MWCNT (indicated by arrow) internalized by macrophages. M-MWCNT was co-cultured with macrophages for 12 h and then the collected cells were immediately fixed with 2.5% glutaraldehyde. The fixed cells were sectioned and observed by TEM. (b) CLSM image of FITC-OVA uptake by RAW267.4. Macrophages were cultured with M-MWCNT+FITC-OVA or FITC-OVA for 24 h. The slides were fixed and stained with DAPI as indicated by blue fluorescence, while green fluorescence indicates FITC-OVA. The cells were observed and photographed with a laser confocal scanning microscope.