Figure 7.

Effects of M-MWCNT on the maturation of DCs in ICR mice. The splenocytes suspension were prepared from the spleens of ICR mice 48 h after the first immunization. Point diagram analysis of dendritic cell double-stained with CD11c⁺-MHC-II⁺, CD11c⁺-CD86⁺, and CD11c⁺-CD80⁺. The percentage of CD80⁺, MHC II⁺, and CD86⁺ cells against total DCs was determined by using flow cytometry. Distribution map of CD11c⁺-MHCII⁺, CD11c⁺-CD86⁺, and CD11c⁺-CD80⁺ co-expressing cells in Supplementary Materials. The data are presented as the mean ± SD (n = 3) analyzed by one-way ANOVA and then Duncan’s multiple-range tests. Different letters (a, b, c, and d) above each group of bars indicate statistically significant differences (p < 0.05).