Figure 8.

The M-MWCNT increased the proliferation of splenocytes and M-MWCNT the secretion of cytokines. The total splenocytes were isolated from the spleen of ICR mice in all groups on days 7, 14, 21, and 28 after the last immunization. The splenocyte proliferation rate (a,b) was estimated by the CCK-8 method. LPS and Con A, used as a mitogen, were co-cultured with splenocytes. The proliferation activity is represented as proliferation rate (%). The serum concentrations of IFN-γ (c) and IL-6 (d) in ICR mice were measured using ELISA. The data are presented as the mean ± SD (n = 3) analyzed by one-way ANOVA and then Duncan’s multiple-range tests. Different letters (a–f) above each group of bars indicate statistically significant differences (p < 0.05).