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. 2022 Apr 18;11(4):613. doi: 10.3390/biology11040613

Figure 2.

Figure 2

Analysis of the WT and the p.V781I and p.A1737T mutant sodium current inactivation process. (A) Current traces were recorded in the WT and the mutant p.V781I and p.A1737T Nav1.4 channels in response to a brief +10 mV test pulse preceded by a conditioning pulse in a two-pulse voltage protocol (see Section 2 for more details). (B) Voltage dependence of the quasi-steady-state inactivation. Peak sodium currents in response to the +10 mV test pulse are plotted as a function of the conditioning potential, evoked in a 5 mV stepwise escalating from −140 to +40 mV (n = 10). (C) The half-inactivation voltage, V1/2, of Nav1.4 channels was approximately −66.0 ± 0.7 mV, −59.1 ± 0.8 mV, and −58.5 ± 0.9 mV for the WT, p.V781I, and p.A1737T channels, respectively (n = 10). (D) The cumulative data that slope (k) of the Nav1.4 channel for the WT, p.V781I, and p.A1737T mutant channels. Data are expressed as means ± SEM (* p < 0.05, n = 10; N.S., no statistically significant difference compared to control).