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. 2022 Apr 10;27(8):2438. doi: 10.3390/molecules27082438

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Binding of DNA duplexes to MutS(A469C). (a) Binding efficiency. Formation of the MutS(A469C) complex with an unmodified G/T-containing duplex is set to 100%. The standard deviation from the mean of at least three experiments is indicated; p < 0.05. (b) Analysis of complex formation between MutS(A469C) and ³²P-labelled duplexes 17GT-dU_s^ethynyl-8, 17GT-dU_m^ethynyl-8, 17GT-dU_l^ethynyl-8 (top panel), 17GT-dU_s^ethynyl-11, 17GT-dU_m^ethynyl-11 and 17GT-dU_l^ethynyl-11 (bottom panel) by an electrophoresis mobility shift assay (EMSA). DNA concentration was 0.5 µM. MutS(A469C) concentration was 1 µM (a, lanes 1) or 2.5 µM (lanes 2). Lanes K correspond to DNA without the protein. Autoradiograph of a 6% polyacrylamide gel.