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MutS(N469C) (a) or MutS(N497C) (b) cross-linking with 17GT-dUsacryl-5, 17GT-dUmacryl-5 or 17GT-dUlacryl-5 (lanes 1–3); 17GT-dUsacryl-8, 17GT-dUmacryl-8 or 17GT-dUlacryl-8 (lanes 4–6); or 17GT-dUsacryl-11, 17GT-dUmacryl-11 or 17GT-dUlacryl-11 (lanes 7–9). Conjugate yields (%) are indicated under the gel lanes. Protein concentration on the monomer basis was 5 µM, DNA: 0.5 µM. Duplexes contain a 32P label at the 5′ end of the modified strand. Autoradiograph of SDS-PAGE in an 8% gel.
