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. 2022 Apr 10;27(8):2438. doi: 10.3390/molecules27082438

Figure 7.

Figure 7

Analysis of MutS(N497C) (5 μM on the monomer basis) cross-linking with the 17GT-dUmacryl-8 DNA duplex (0.5 μM) by SDS-PAGE in an 8% gel. Reaction conditions: 0–120 min, 37 °C. Conjugate yields (%) are indicated under the gel lanes. The gel was stained with a Coomassie G250 solution. M: markers of protein molecular mass, kDa.