Figure 8.
Inhibition of the proteasome does not lead to stabilization of Alys: (A–D) Whole-cell extracts from cells expressing (A) Aly1-GFP, (B) Aly1PPXYless-GFP, (C) Aly2-GFP, or (D) Aly2PPXYless-GFP were treated with either vehicle control (DMSO) or MG132 for 60 min and then cycloheximide (CHX) for the time indicated (hours); proteins were resolved by SDS-PAGE. Anti-GFP antibody was used to detect tagged α-arrestins and REVERT total protein stain was used as a loading control. Blots shown are one representative from 2–3 replicate experiments. Molecular weights are shown on the left side in kDa.