Table 1.
Growth and EPS parameters.
| STRAINS | EPS Production Phase |
Apparent µmax (d−1) | Dt (d) | Final Biomass Production (×106 cell·mL−1) |
Final Biomass Production (g L−1) |
Final RPS Production (g L−1) | Productivity RPS (g EPS/g DW Biomass) |
Productivity RPS (mg EPS/106 cell) |
Productivity BPS (g EPS/g DW Biomass) |
|---|---|---|---|---|---|---|---|---|---|
| T. oligopyrenoides | Stationary | 0.152 | 4.55 | 31.8 | 2.2 | 1.12 | 0.35 | 0.035 | nd |
| E. coxiae | End of Log/ Stationary |
0.2632 | 2.63 | 28.34 | 2.04 | 1.43 | 0.47 | 0.05 | nd |
| E. madagascarensis | End of Log/ Stationary |
0.1026 | 6.75 | 20.9 | 1.79 | 1.63 | 0.78 | 0.078 | nd |
| P. sordidum | Log/ Stationary |
0.151 | 4.58 | 28.2 | 1.81 | 1.91 | 0.68 | 0.068 | nd |
| N. cyanea | End of Log/ Stationary |
0.172 | 4.02 | 15.4 | 2.35 | 2.32 | 0.99 | 0.151 | nd |
| C. japonica | Log/ Stationary |
0.067 | 10.34 | 6 | 2.49 | 1.59 | 0.64 | 0.265 | nd |
| C. ornatum | nd | nd | nd | nd | 1.2 | 0.014 | 0.0086 | nd | 0.24 |
| C. richteriana | nd | nd | nd | nd | 0.64 | 0.023 | 0.0018 | nd | 0.19 |
| B. subsimplex | nd | nd | nd | nd | 0.46 | 0.011 | 0.0013 | nd | 0.08 |
| R. sordida | nd | nd | nd | nd | 0.72 | 0.021 | 0.0038 | nd | 0.14 |
| R. brevistipitata | nd | nd | nd | nd | 0.51 | 0.013 | 0.0021 | nd | 0.11 |
RPS: Released polysaccharide (EPS free in the culture medium); BPS: Bound polysaccharide (EPS bound to the cells and extracted as described in Materials and Methods section). Final biomass (g L−1) was determined by dry weight measurement after centrifugation and recovery of the whole biomass. Final EPS concentration (g L−1) is obtained after extraction and purification of the supernatant, and corrected by the purity of the extract, determined by total sugar assay. Dt: doubling time, nd: not determined.