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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2364:25–52. doi: 10.1007/978-1-0716-1661-1_2

Fig. 3.

Fig. 3

PREM of the cytoskeleton of a cultured PtK1 potoroo epithelial cell after depletion of actin filaments by gelsolin treatment. Image shows a peripheral cell region containing two large focal adhesions (arrows). Abundant myosin II filaments, a subset of which is highlighted in yellow, accumulate along the actin-depleted stress fibers extending away from focal adhesions, while some individual myosin II filaments can be seen elsewhere in the cytoplasm. Exposed microtubules (red), intermediate filaments (blue), and clathrin-coated vesicles (green) are also visible. Scale bar: 500 nm