Figure 6.

Effect of EPO and TAM, alone or in combination, on apoptosis in MCF-7 cells: (A–E) Dot-plots from flow-cytometric analysis of cell apoptosis using annexin V-FITC/PI dual-staining in MCF7 cells. Cells in the upper-left quadrant are necrotic, cells in the upper-right quadrant have late apoptosis, the lower-left quadrant has normal living cells, and the cells in the lower-right quadrant are early apoptotic cells; (F) the bar chart describes the total percentage of apoptosis in MCF7. Results are shown to be significant at p < 0.05. EPO: evening primrose oil; TAM: tamoxifen; φ: is significant from the control group; ≠: is significant from the EPO group; *: is significant from the TAM group; θ: is significant from the combination-1 group.