Figure 2.

Intracellular zinc flux assay of HeLa cells stably expressing wild-type and variant TMEM163 proteins. The HLD-linked variant TMEM163-L76P showed significantly enhanced zinc efflux, while the TMEM163-L76R variant displayed marked loss of zinc efflux activity that mimicked the inactive TMEM163-D124A-D128A mutant. Unmodified HeLa cells and stable cells expressing the TMEM163-E286K mutant were included as additional controls. Significance testing was performed using ANOVA with repeated measures followed by a post-hoc analysis using Tukey’s multiple comparisons test (p < 0.0001, n = 4 independent trials). Data are represented as means ± SEM. A.U., arbitrary unit.