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Efficient tissue homogenization using micropestles. (A) Total protein extracted from 1 mm cores with a dry-weight < 1 mg (n = 5; unpaired t-test; p = 0.70). (B) Intra-method %CVs based on all quantified proteins, median %CV are given. (C) Pearson correlation plot based on all quantified proteins. (D) Volcano plot highlighting proteins that were significantly enriched by one method (multiple hypothesis testing using the FDR-based approach by Benjamini–Krieger, FDR 1%).
