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. 2022 Apr 18;23(8):4443. doi: 10.3390/ijms23084443

Figure 4.

Figure 4

An SDC–TCEP-based buffer improves overall protein recovery from FFPE tissues. (A) Total protein extracted from 1 mm cores with dry-weight < 1 mg (n = 5; unpaired t-test; p = 0.0341). (B) Intra-method %CVs based on all quantified proteins, median %CVs are given (unpaired t-test; p < 0.0001). (C) Pearson correlation plot based on all proteins quantified with both methods. (D) Volcano plot highlighting proteins that were significantly enriched by either method (multiple hypothesis testing using FDR-based approach by Benjamini–Krieger, FDR 1%).