Figure 1.

Representative monocyte subsets’ gating strategy in peripheral blood (PB) of COVID-19 patients. (a) FSC-A vs. FSC-H plot: gating the cells that have an equal area and height, thus removing clumps (greater FSC-A relative to FSC-H) and debris (very low FSC). (b) SSC-A vs. CD45 plot: selection of monocytes based on their SSC+/CD45+bright properties. (c) SSC-A vs. HLA-DR plot: selection of monocytes based on their SSC+/HLA-DR+bright properties. (d) CD14 vs. CD16 plot: to gate the monocyte subsets—classical (blue), intermediate (turquoise) and non-classical (pink) (the exact antigenic characterization can be found in the text in Section 2, Flow cytometry analysis).