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. 2022 Apr 15;27(8):2563. doi: 10.3390/molecules27082563

Figure 3.

Figure 3

HSMM differentiation into multinucleated myotubes. Representative phase contrast (a) and dark-field images (b) of MYH2-immunostained cells (green), with Hoechst-labeled nuclei (blue) after 1 day growth medium (phase contrast image, (a)) and 3 days in the differentiation medium (dark-field image, (b)). (Images at ×20 magnification; scale bar = 100 microns).