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. 2022 Apr 15;27(8):2563. doi: 10.3390/molecules27082563

Figure 4.

Figure 4

Skeletal muscle cell marker gene expression by RT-PCR. Plots show gene expression of late muscle cell markers Myosin heavy chain-2 (MYH2), Troponin T (TNNT1) and Myogenin (MYOG), normalized to three most stable reference genes, eEF1a, RPL13a and B2M, (Avg M value = 0.269) and then the 2−ΔΔCt algorithm was applied for relative quantification. *** p < 0.001 vs. day 1 using Student’s t-test.