TABLE 4.

Reproducibility of MAGIC-5 assay with NL432

Drug	Repetition no.a	IC50 (μM)					Overall mean ± SD	CVb(%)
		1c	2	3	4	5
AZT	1	0.010	0.012	0.030	0.056	0.030
	2	0.036	0.010	0.044	0.030	0.042
	3	0.028	0.011	0.045	0.054	0.074
	Mean	0.025	0.011	0.040	0.047	0.049	0.034 ± 0.02	46
3TC	1	1.20	1.20	0.52	0.26	1.20
	2	0.40	0.42	1.00	0.58	0.42
	3	1.80	1.20	0.86	0.70	0.85
	Mean	1.13	0.94	0.79	0.51	0.82	0.84 ± 0.23	27
d4T	1	2.1	1.4	2.6	2.2	2.6
	2	2.3	2.2	1.8	2.0	1.3
	3	1.9	2.3	1.4	1.2	2.1
	Mean	2.10	1.97	1.93	1.80	2.00	1.96 ± 0.11	6
RTV	1	0.028	0.027	0.010	0.090	0.010
	2	0.021	0.013	0.100	0.060	0.013
	3	0.030	0.024	0.022	0.042	0.046
	Mean	0.026	0.021	0.044	0.064	0.023	0.03 ± 0.02	51
SQV	1	0.0010	0.0010	0.0024	0.0010	0.0076
	2	0.0044	0.0020	0.0042	0.0032	0.0086
	3	0.0056	0.0062	0.0043	0.0040	0.0012
	Mean	0.0037	0.0031	0.0036	0.0027	0.0058	0.0038 ± 0.001	31
NFV	1	0.0028	0.0029	0.0038	0.0032	0.0021
	2	0.0015	0.0025	0.0023	0.0025	0.0025
	3	0.0022	0.0011	0.0012	0.0022	0.0020
	Mean	0.0022	0.0022	0.0024	0.0024	0.0022	0.0023 ± 0.0001	5

^aTo examine reproducibility of the assay, experiments were repeated in triplicate five times. 

^bCV, coefficient of variation. 

^cExperiment number.