Figure 4.

Effect of ostarine on C2C12 differentiation. mRNA expression of myogenin, myh, and myoD after (A–C) 2 days and (D–F) 6 days of differentiation initiation. (G,H) Effect ostarine on myogenin and MyH protein level changes after 2 and 6 days of differentiation initiation and quantification of the amount of proteins in day 2 and day 6 (I,J). Statistically significant differences are represented as * p < 0.05 and ** p < 0.01 versus corresponding control using one-way ANOVA followed by Dunnett’s post hoc test.