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. 2022 Apr 10;23(8):4193. doi: 10.3390/ijms23084193

Figure 4.

Figure 4

Effects of NI-hADSC-CM on Akt and ERK signaling. SH-SY5Y cells were treated for different timepoints with or without ROT (0.5 μM) and assessed by Western blotting (a). SH-SY5Y cells were incubated in the absence or presence of ROT (0.5 μM) for 48 h and then treated with hADSC-CM or NI-hADSC-CM (at 50% dilution each) during the last 24 h. The levels of p-/t-Akt (b), p-/t-ERK1/2 (c), p-/t-GSK3β (d), and beclin-1 (e) protein expression were assessed using the Western blot assay. Original uncut Western blot images are shown in Supplementary Figure S5. Data are presented as the mean ± SEM of three independent experiments. Statistical analysis was performed using one-way analysis of variance followed by Tukey’s post hoc test. Statistical significance: a—compared with control; b—compared with ROT; * p < 0.05, ** p < 0.01, and *** p < 0.001. p-, phosphorylated; t-, total.