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. 2022 Apr 10;23(8):4193. doi: 10.3390/ijms23084193

Figure 5.

Figure 5

Effects of NI-hADSC-CM on LC3B and p62 protein expressions. SH-SY5Y cells were treated for different timepoints with or without ROT (0.5 μM) and Triton X-100-soluble cell lysate fractions assessed by Western blotting (a). SH-SY5Y cells were incubated in the absence or presence of ROT (0.5 μM) for 48 h and then treated with hADSC-CM or NI-hADSC-CM (at 50% dilution each) during the last 24 h. The levels of LC3B (b,c) and p62 (d,e) proteins in Triton X-100-soluble (b,d) and Triton X-100-insoluble (c,e) cell lysate fractions were assessed using the Western blot assay. Original uncut Western blot images are shown in Supplementary Figure S6. Data are presented as the mean ± SEM of three independent experiments. Statistical analysis was performed using one-way analysis of variance followed by Tukey’s post hoc test. Statistical significance: a—compared with control; b—compared with ROT; * p < 0.05, ** p < 0.01, and *** p < 0.001.