Table 1.
Subcellular localization of PvLEA proteins in fusion with AcGFP1 in different cell cultures and WoLF PSORT predictions. We predicted the types of subcellular localization of different PvLEA proteins using WoLF PSORT and obtained experimental data with corresponding PvLEA(x)–AcGFP1 (C-terminal) or AcGFP1–PvLEA(x) (N-terminal) chimeras in Pv11 and Sf9 cells (x = 1–27). N/A—the PvLEA24–AcGFP1 chimera caused aberrations in cell shape and toxicity in Sf9 cells.
| Protein | WoLF PSORT * | CHO ** | Pv11 | Sf9 | ||
|---|---|---|---|---|---|---|
| C–Terminal AcGFP1 | N–Terminal AcGFP1 | C–Terminal AcGFP1 | N–Terminal AcGFP1 | C–Terminal AcGFP1 | ||
| PvLEA1 | ER | Cell membrane | ||||
| PvLEA2 | Nucleus | Cytosol and nucleus | ||||
| PvLEA3 | ER | Possibly ER | ER/ membrane |
ER | ER/ membrane |
Cytosol |
| PvLEA4 | Nucleus | Cytosol and nucleus | ||||
| PvLEA5 | Cytosol | |||||
| PvLEA6 | Cytosol | Cytosol | Cytosol and nucleus |
Cytosol | Cytosol and nucleus |
Cytosol |
| PvLEA7 | Nucleus | Cytosol and nucleus |
||||
| PvLEA8 | Cytosol | Cytosol and nucleus |
||||
| PvLEA9 | Cytosol and nucleus |
|||||
| PvLEA10 | Nucleus | |||||
| PvLEA11 | Cytosol | |||||
| PvLEA12 | ||||||
| PvLEA13 | ||||||
| PvLEA14 | ||||||
| PvLEA15 | ||||||
| PvLEA16 | ||||||
| PvLEA17 | Mitochondria | |||||
| PvLEA18 | Cytosol | |||||
| PvLEA19 | Cytosol | Cytosol and nucleus |
||||
| PvLEA20 | Nucleus | |||||
| PvLEA21 | Cytosol | Cytosol | ||||
| PvLEA22 | ER | |||||
| PvLEA23 | Nucleus | Cytosol | ||||
| PvLEA24 | Cytosol | N/A | ||||
| PvLEA25 | Cytosol and nucleus |
Cytosol and nucleus |
||||
| PvLEA26 | ||||||
| PvLEA27 | ||||||
* Data on WoLF PSORT predictions of PvLEA localization were reproduced using the contemporary version of the WoLF PSORT server (https://wolfpsort.hgc.jp/, accessed on 18 March 2022) and are mostly in accordance with predictions in [13]. Indicated localization types are predictions with the highest WoLF PSORT score; a detailed report with all predictions is attached in Table S3. ** Data on PvLEA localization in CHO cells are in accordance with [13] and were obtained for C-terminal fusions only.