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. 2022 Apr 16;23(8):4419. doi: 10.3390/ijms23084419

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of acid acclimation on the HA accumulation in the plasma membranes of the gills. The protein expression of HA in the branchial plasma membranes acclimated to acid environments was analyzed by Western blot. The blots showed the band with a molecular weight corresponding to Atp6v1a (approximately 70 kDa), and the PVDF membrane stained by ponceau S showed bands of total loading proteins (a). The protein expression of HA was quantified and normalized to total loading protein (N = 6) (b). Values are the mean ± SD. Student’s t-test, ** p < 0.01.