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. 2022 Apr 11;13(4):669. doi: 10.3390/genes13040669

Figure 2.

Figure 2

METTL3-D is downregulated in HCC and correlates with tumor stage. (A) mRNA levels of METTL3-A, -B, -C, and -D in normal and HCC tissues from the GEPIA2 database. (B) RT–qPCR analyses of the mRNA levels of METTL3-A, -B, -C, and -D in NATs and tumor tissues from our patients. The clinical characteristics of all the patients are listed in Table S1. (C) mRNA levels of METTL3-A and METTL3-D are correlated with tumor stage. The mRNA levels were analyzed by RT–qPCR, and patients were grouped as early-stage HCC (Stage I) and late-stage HCC (Stage III). (D) The protein levels of METTL3-A were upregulated in HCC tumor tissues, while METTL3-D was downregulated. Right, Western blot analysis of Flag-tagged METTL3 variants on the pcDNA3 vector in HepG2 cells using the anti-FLAG antibody. Left, Western blot analysis of the endogenous METTL3 variants in HCC patient NATs and tumor tissues using the anti-METTL3 antibody. (E) Information concerning patients in panel (D). (F) The protein levels of METTL3-A and METTL3-D in patients were correlated with their tumor stages. The data are expressed as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (t test). Black and red asterisks have the same meaning.