METTL3-D inhibits the proliferation, migration, and invasion of HCC cells. (A) Expression of METTL3-D decreases the proliferation rate of HCCLM3 cells. Left, CCK-8 assay analysis; right, cell counting assay analysis. (B) The MTT assay showed that METTL3-D expression decreases the proliferation rate of HCCLM3 (left) and HepG2 (right) cells. (C) Expression of METTL3-D inhibits the migration of MHCC97L cells. Representative images are shown at 0 h and 48 h after transfections. (D) Expression of METTL3-D inhibits the invasion ability of MHCC97L cells. The data are expressed as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (t test).