Figure 5.

Cd exposure increased heterogeneity in nuclear size. The nuclear size, shape and migration tracks are shown. (A). ht-UTLM6. (B). CR-LM6. Scale bar = 20 μm. (C,D). Scatterplots to show the distribution of nuclear size over time. Note the presence of large nuclei on top of the plots. The five colored curves correspond with median (red), 25% and 75% quantiles (gold) and 10% and 90% quantiles (green). (C). ht-UtLM6. (D). CR-LM6. As shown by the blue dashed line, in ht-UtLM6 there was no nuclear objects with a size greater than 300 µm², whereas in CR-LM6 there were 190 nuclear objects with a size greater than 400 µm². (E). Medians of nuclear size at each time point were plotted against frame number. (F). Variances of nuclear size at each time point were plotted against frame number.